Coding

Part:BBa_K5136003:Design

Designed by: Xiaoxiao Zhang   Group: iGEM24_XMU-China   (2024-09-14)


CYP199A4 T253A-His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 150
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 831
    Illegal XhoI site found at 1231
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 150
    Illegal AgeI site found at 691
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

synthesis

References